Department of Biomedicine and Prevention
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Laboratory of Human Anatomy

(Responsabili Prof. Pellegrino Rossi – Prof. Susanna Dolci) ​
Picture

The research activity of the laboratory focuses on two main topics: Cancer Biology and Germ Cells Biology.
  • Cancer Biology. The laboratory goal is to identify the molecular mechanisms of cancer etiology, progression and treatment with a main focus on the study of testicular, prostate, bladder cancers and tumors of the nervous system.
  • Germ Cells Biology. The research in this field currently focuses on the study of the mechanisms of chromosome segregation during meiosis and regulation of spermatogenesis by cannabinoids.
 
STUDY APPROACHES:
 
  • Molecular Biology Techniques:
  • DNA Biology and manipulation: DNA recombination assays, cloning and in situ mutagenesis.
  • RNA Biology: semiquantitative and quantitative PCR; genome-wide analysis of RNA (RNAseq), UV- Cross-linking and Immunoprecipitation (CLIPseq) and Chromatin Immunoprecipitation (ChIPseq)-sequencing datasets.
  • In silico analysis of public datasets,  in vivo splicing and 3’RACE assays.
  • Analyses of nascent RNA transcripts and RNA polymerase II processivity.
  • Protein Biology: western blot, transient and stable depletion/overexpression of selected genes, luciferase assay, Protein-DNA (ChIP), Protein-RNA (RNA-pulldown, CLIP, RNA immunoprecipitation), and Protein-Protein (Protein gradient fractionation, yeast and mammal two-hybrid, GST-protein pulldown, Co-immunoprecipitation) interaction. Luciferase assay.
 
  • Cell Biology and histology techniques:
Cell culture, Proliferation, clonogenic, invasion and chemotherapy response assays, Flow cytometry, immunofluorescence, organ inclusion and H&E staining, immunohistochemistry.
 
  • In vivo studies on animal models:
Animal manipulation from embryonal to adult stage. Generation of Knock-in, knock-out mouse models and their phenotypic characterization. Generation of xenograft models for the in vivo study of cancers progression and response to chemotherapy treatments.
 
RESEARCH FIELDS BY INVESTIGATOR
 
Prof.ssa Susanna Dolci (dolci@uniroma2.it)
Our lab uses genetically engineered mouse models to study human disease. In particular we produced mouse strains to understand the role of MAPK and GMP signaling in the genesis of tumors of the nervous system, of germ cells and of melanocytes. We aim to develop mouse cancer models based on the evidence that mutations of Ras-Raf-Mek signaling pathway strongly impact on cell transformation and to transfer our findings for validation in human cancer samples.
 
Prof.ssa Pamela Bielli (bllpml01@uniroma2.it)
In humans regulation of gene expression is primarily controlled by regulation of RNA processing, a set of stepwise post-transcriptional modifications responsible of transcriptome expansion and proteome diversity. Aberrant RNA processing regulation can generate dysfunctional proteins leading to modification of fundamental physiological processes and thus raising the risk to develop pathological conditions. Due the relevance of this process, our laboratory is mainly focused on the study of RNA processing regulation in physiological processes, as apoptosis and differentiation, and in pathological condition, as cancer and neurodegenerative diseases. During the last five years, our research work mainly contributed to elucidate the role of pro-oncogenic splicing factors in alternative splicing regulation of cancer-related genes in tumors, including prostate and bladder cancer. Currently, the understanding of molecular mechanisms underlying alternative polyadenylation and transcription termination regulation and their biological relevance in tumors are our main focus.
 
Prof.ssa Paola Grimaldi (p.grimaldi@med.uniroma2.it)
In the last 10 years our laboratory has been focused on the study of the “Endocannabinoid system” (ECS) in germ cells and in CNS. The ECS is ubiquitously expressed and is involved in maintaining cell homeostasis. It is composed of lipid molecules, called endocannabinoids, that bind to cannabinoid receptors CB1 and CB2, and enzymatic proteins that synthetize and degrade endocannabinoids. ECS regulates many biological processes such as proliferation, differentiation, apoptosis and its alteration is associated to pathological conditions. In germ cells we elucidated the role of CB2 receptor in promoting meiotic entry of male and female germ cells and in inducing epigenetic modifications in these cells that can be transmitted to the future generation. In the field of CNS, we demonstrated the effect exerted by CB1 receptor in Neural stem cells differentiation and maturation into neurons. Studies on these fields are currently in progress.
 
Prof. Pellegrino Rossi (pellegrino.rossi@med.uniroma2.it)
 Starting from 1990 and up to date, his main field of research is the study of the mechanisms that control the differentiation of male germ cells, and in particular: expression and function of proto-oncogenes in spermatogenesis, interaction between pituitary hormones and paracrine factors in testicular regulation of hormone-dependent gene transcription, control of meiotic progression, control of gene expression in the haploid phase, genetic mechanisms of male infertility, molecular basis of fertilization, mechanisms of specification of the germ cell line, generation of animal models for testicular germ cell tumors.
 
Dr. Marco Barchi (marco.barchi@uniroma2.it)
Meiosis is the specialized process that in sexually reproducing organisms leads to the differentiation of gametes. Proper segregation of chromosomes during meiosis is key to prevent conception of aneuploid embryos, such as that carrying trisomy of chromosome 21 (Down syndrome) and that of sex chromosomes (Klinefelter and Turner syndromes). Using animal models, our laboratory is interested at understanding the molecular mechanisms behind chromosome recombination and segregation during meiosis. Moreover, using germ cell cancer cell lines of human origin, we are interested at identify the role of DNA recombination in testicular cancer acquired-resistance to chemotherapy.
 
Dr. Flavia Botti (botti@uniroma2.it)
Since 1994 working in a hospital context, initially in the field of Dentistry, later in the field of Anatomical Pathology I collaborated transversally in various research fields. From the study of materials for implantology, to the optimization of preparation techniques for optical and electron microscopy for non-decalcified human dental tissues, to the role of the transcription factor FBI-1 in the regulation of alternative splicing when  it interacts with the splicing factor SAM68. Currently, in collaboration with the orthopedics department, I am involved in the study of the histological characteristics found in discoid lateral menisci of young patients in order to better understand the pathophysiology of that disease.
 
PUBLICATIONS (Pub Med):
 
Prof. Susanna Dolci
https://pubmed.ncbi.nlm.nih.gov/?term=dolci+susanna&sort=date&size=20
 
Prof.ssa Pamla Bielli
https://pubmed.ncbi.nlm.nih.gov/?term=pamela+bielli&sort=date&size=20
 
Prof.ssa Paola Grimaldi
https://pubmed.ncbi.nlm.nih.gov/?term=grimaldi+paola&sort=date&size=20
 
Prof. Pellegrino Rossi
https://pubmed.ncbi.nlm.nih.gov/?term=pellegrino+rossi&sort=date&size=20
 
Dr. Marco Barchi
https://pubmed.ncbi.nlm.nih.gov/?term=barchi+marco&sort=date&size=20
 
Dr.ssa Flavia Botti
https://pubmed.ncbi.nlm.nih.gov/?term=Botti+flavia&sort=date&size=20
​
STAFF
​Prof. Susanna Dolci
Prof. Pamela Bielli
Prof. Paola Grimaldi
Prof. Pellegrino Rossi
Dr. Marco Barchi
Dr. Flavia Botti

​Dipartimento di Biomedicina e Prevenzione
Università di Roma Tor Vergata 

presso Facoltà di Medicina e Chirurgia
Indirizzo:  Viale Montpellier, 1 – Roma
Direttore del Dipartimento
Prof. Antonio De Lorenzo


Responsabile Amministrativo
Avv. Ombretta Picchioni


Content manager del sito
Prof. Andrea Malizia
  • Homepage
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    • Specialization School in Nutrition Science Università Cattolica Nostra Signora del Buon Consiglio
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    • Laboratory for the Prevention and Management of Psychosocial Risks (stress, mobbing and burn out)
    • Laboratory of Environmental and Occupational Toxicology
    • Laboratorio of Human Anatomy
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    • Sezione di Nutrizione clinica e Nutrigenomica
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